To assess the therapeutic significance of MCB1 in HCC with drug resistance in vivo, an orthotopic (sub‐capsular space of the liver) xenograft model established using HCCLM3SR‐luc and Huh7LR‐luc cells was administrated with an adeno‐associated virus (AAV) targeting MCB1. The gene discussed is PSMD4; the disease is hepatocellular carcinoma.