CHL1 and neoplasm: Spatially, transcriptomics data revealed that signaling related to immune and ECM regulation, such as the adaptive immune system and degradation of the ECM, as well as cell adhesion, like neural cell adhesion molecule (NCAM) signaling for neurite outgrowth, were among the most affected signaling downregulated due to Gal-1 silencing found at the tumor margin (Supplemental Fig. 2A).