The gross and spatial microscopy images of a healthy liver, MAFLD, HCC, and MASH-HCC are shown in Fig. 2A. Figure 2A also provides an example of selecting a region of interest (ROI) and further segmentation to select the area of interest illumination (AOI) based on CD45 (red) or pan-cytokeratin (green) and SYTO13 nuclear stain (blue) markers. The gene discussed is PTPRC; the disease is hepatocellular carcinoma.