EGFR and Pleural effusion: With respect to the specimen of pleural effusion, the results revealed that (1) EV-DNA- and cfDNA-based EGFR genotyping for patients who were either EGFR-TKI naïve without EGFR-TKI treatment or who acquired resistance to EGFR-TKI also showed good agreement with tissue biopsy; (2) the detection rate of p.T790M using EV-DNA was more efficient than that using cell blocks or cfDNA [126].