Moreover, comparing 30 CBAVD patients with CFTR gene mutations and 112 non-CBAVD obstructive azoospermia patients undergoing ICSI with testicular sperm extraction, we observed that although the 2PN rate was significantly higher in non-CBAVD obstructive azoospermia, there were no significant differences in fertilization rates, pregnancy rates, miscarriage rates, and live birth rates. This evidence concerns the gene CFTR and Azoospermia.