Further CD34 immunohistochemical analysis (Fig. 4a) showed that the first layer of the keloid had a large number of blood vessels with open lumens; the number of blood vessels gradually decreased from the first layer downwards (Fig. 4b), and the lumens were gradually closed; the second layer of blood vessels had partially open lumens; the third layer of the keloid had the smallest number of blood vessels with completely closed lumens. The gene discussed is CD34; the disease is keloid.