The CCM2-depleted iPSC-derived ECs exhibited increased amounts of actin stress fibers when compared with wild-type cells (Fig. 3J,K) and a weakened VE-cadherin labeling, indicative of weakened cell adhesion (Fig. 3L,M), which are two hallmark features of the CCM pathology. This evidence concerns the gene CDH5 and cerebral cavernous malformation.