In this study, in vitro experiments on A549 cells and H1299 cells confirmed that oleic acid can inhibit the expression of SDC4, promote the occurrence of ferroptosis in A549 cells and H1299 cells through the GPX4/ACSL4 pathway, and provide an effective basis for the clinical application of targeted ferroptosis drugs in the effective treatment of lung cancer. Here, GPX4 is linked to lung cancer.