Although Notch2 pathway is the most activated homologous protein in these GBM cells (Fig. S2a), relevance of inhibiting both pathways (Notch2 and Notch3) to downregulate Hes1 protein was also determined when targeting Notch2 or Notch3 relative to non-specific siRNA transfected cells (Fig. S2e). The gene discussed is HES1; the disease is glioblastoma.