Although overexpression of Notch3 has been reported in 71% of primary GBM associated with aberrant HES1 expression [6, 46], our data indicates that Notch2 signaling is the predominant pathway in these GBM cell lines, as supported by The Human Protein Atlas database (proteinatlas.org/NOTCH2 for NOTCH2 and proteinatlas.org/NOTCH3 for NOTCH3). The gene discussed is HES1; the disease is glioblastoma.