While previous literature has reported the use of anti-Vimentin monoclonal antibodies and other markers for diagnosing osteosarcoma in rabbits, this study marks the first utilization of Runx-2, a crucial transcription factor in cartilaginous and bone cells, as a target for immunolabeling and diagnosis (Thompson & Pool, 2002; Wijesundera et al., 2013). This evidence concerns the gene RUNX2 and osteosarcoma.