KEGG enrichment analysis also suggested that DEGs were involved in the regulation of the Regulation of actin cytoskeleton (disulfidptosis can affect this regulation process), as well as pathways related to colon cancer development, including the PI3K-Akt signaling pathway, NF-kappa B signaling pathway, TGF-beta signaling pathway, and pathways related to cell signaling, immune response, cell adhesion, and apoptosis (Figures 3C, D). Here, AKT1 is linked to colonic neoplasm.