Furthermore, we constructed wild-type and mutant vectors of the <i>ABCD1</i> (NM0000334) gene to validate the effect of this mutation on the expression of the <i>ABCD1</i> gene and protein and to explore the mechanism of X-linked adrenoleukodystrophy occurrence and development to identify therapeutic targets for clinical treatment. Here, ABCD1 is linked to X-linked adrenoleukodystrophy.