GSEA enrichment results in PCOS showed that highly expressed FAM166B was mainly enriched in signaling pathways such as Insulin secretion, Fructose and mannose metabolism, Regulation of lipolysis in adipocytes, Inflammatory mediator regulation of TRP channels, Carbohydrate digestion and absorption, Steroid biosynthesis and Platelet activation (Figures 7E, F). This evidence concerns the gene INS and polycystic ovary syndrome.