• PNX treatment in obese infertile rats leads to significant improvements in various parameters. It decreases serum levels of insulin and testosterone, as well as ovarian levels of dynamin-related protein 1, reactive oxygen species, TNF-α, MDA, and caspase-3.• PNX treatment also increases serum levels of estrogen, progesterone, LH, and FSH, along with ovarian levels of GnRH receptor, mitofusin2, mitochondrial transmembrane potential, and electron transport chain complex-I. Here, GNRHR is linked to Infertility.