The data suggests that Twist1 expression influencesmatrix remodelling in a cell specific manner, as the trends for geneupregulation with matrix stiffening are only present in 786-O cultures.Although the pathological significance is still unclear, there ispotential clinical value in tracking Twist1 expression, as it canindicate tumor aggressiveness in RCC patient tissue samples.75 Here, TWIST1 is linked to renal cell carcinoma.