Related genes (Nvd, Spo, Phm, Dib, Sad, and Shd) for synthesis of 20E were regulated, and CYP18A1, an enzyme transforming 20E to 20,26-dihydroxyecdysone, was also enriched (Figure 3A), indicating the activation of this pathway in the larvae after fungal infection. The gene discussed is PAM; the disease is fungal infectious disease.