Using the Nakagawa CKD Kidney Dataset (Nakagawa et al. 2015), which comprises of microarray gene expression profiles from 53 biopsy specimens of CKD and HC patients, the expression values of both NSA2 and GFM2 were found to be significantly higher in CKD group than in the HC group (NSA2 p < 0.001, GFM2 p < 0.0001, Fig. 3a), and the regulation trend of NSA2 and GFM2 were similar as indicated by the heatmap (Fig. 3b). This evidence concerns the gene NSA2 and chronic kidney disease.