TRIM29-knockdown HT-29 IECs produced more than 2-fold amount of IFN-λ3 (Fig. 1D and 1G) and IL-18 (Fig. 1Eand 1H) compared to the control (sh-Ctrl) HT-29 IECs upon infection with enteric RNA virus rotavirus (Fig. 1D and 1E) or EMCV (Fig. 1G and 1H), indicating TRIM29 inhibits IFN-λ3, and IL-18 production in human HT-29 IECs following enteric RNA virus infection. Here, IFNL3 is linked to infection.