To further determine whether the expressions of TRIM29, NLRP6 and NLRP9b were regulated during enteric RNA virus infection in vitro, we infected primary IECs from mouse intestine organoids of Trim29fl/fl and Trim29IEC-KO mice with EMCV or Rotavirus, respectively, and then measured the expressions of TRIM29, NLRP6 and NLRP9b in IECs after virus infection. Here, NLRP6 is linked to viral infectious disease.