Flow cytometry analysis showed comparable frequencies and cell numbers of intraepithelial Ly6A+CCR9+CD4+ T cells in small intestines from Trim29fl/fl and Trim29IEC-KO adult mice (Fig. 5A and 5B) before EMCV infection or from Trim29fl/fl and Trim29IEC-KO suckling mice (Supplementary Fig. 6A) before rotavirus infection. This evidence concerns the gene LY6S and infection.