To determine whether the loss of this chromosomal looping would change MYL12B transcription in retinoblastoma cells, we measured the expression of MYL12B. We found that the expression of MYL12B at both the RNA (Figure 4H) and protein (Figure 4I) levels was significantly lower in tumor cells than in normal ARPE19 cells. The gene discussed is MYL12B; the disease is retinoblastoma.