Moreover, similar as 3xTg-AD mice, after treatment of IsoLiPro to 10-week-old 5xFAD mice for 12 weeks, the IHC staining (Fig. EV5A–D) and Western blotting (Fig. EV5E–G) results also revealed that IsoLiPro treatment reduced USP11, total tau and p-tau levels in 5xFAD mice. The gene discussed is MAPT; the disease is Alzheimer disease.