HES6 and neoplasm: NE-1, whose leading genes include coordinated expression of Ascl1 and other TFs implicated in neuronal biology43 (Hes6, Prox1 and Insm1), was enriched across all NEPC regions and corresponded to regions with high Mycn and Olig3 expression as well as KRT8+;ASCL1+ tumor cells (Fig. 3b and Supplementary Fig. 3).