We found a consistent trend, showing an inverse relationship between BAG3 and PHF1 levels at single cell levels, i.e., cells with accumulation of ptau have significantly reduced BAG3 compared to cells without ptau accumulation (Fig. 4e–h, Supplementary Fig. 6, Fig. 5d–g, and Supplementary Fig. 7) although global BAG3 increased in animal models and human post-mortem brain tissues with TBI and AD (Supplementary Fig. 8). This evidence concerns the gene BAG3 and Alzheimer disease.