To delineate whether the increased glutamine levels at steady-state are because of decreased glutamine use or increased flux, we performed a metabolic tracing experiment where WT and MIC26 KO cells, cultured in normoglycemia and hyperglycemia, were fed with labelled glutamine [U-13C5, 15N2] for 0.5 and 6 h (Fig 6E–O). The gene discussed is APOO; the disease is Hyperglycemia.