Despite the heterozygous nature of KCNT1 GOF variants in the overwhelming majority of KCNT1-related DEE patients, research efforts, including our own, have largely focused on homozygous GOF variant effects on channel function and neuronal physiology (Gertler et al., 2019; Kim et al., 2014; McTague et al., 2018; Mikati et al., 2015; Milligan et al., 2014; Quraishi et al., 2019; Shore et al., 2020; Tang et al., 2016). This evidence concerns the gene KCNT1 and developmental and epileptic encephalopathy.