AKT1 and breast cancer: On the one hand,the phosphorylated and activated AKT/PKB mediates the stimulatoryeffect of estrogen on breast cancer cells by inhibiting GSK3β,leading to the activation of the Wnt-β-catenin pathway.66 In line with this expected effect, our GSEAanalysis revealed that the abundance of the proteins related to theWnt-β-catenin pathway was enriched in E2-treated MCF7 cellscompared to vehicle-treated control cells (Table 2).