Here, we present the wider result from our RNAi screening and functional studies, indicating that knockdown of other G2/M checkpoint kinases (PLK1, AURKA, TPR, and TTK), and even structural kinetochore components (NUF2 and NDC80) or microtubule subunit tubulin (TUBA1B), can also inhibit TNFα-induced NF-κB reporter gene activity, RELA nuclear translocation, and cell viability in HNSCC. This evidence concerns the gene PLK1 and head and neck squamous cell carcinoma.