In addition, the fecal microbiome of treated mice was characterized using 16S rRNA amplicon sequencing.<h4>Results</h4>HLJD decoction at doses of 2 and 4 g/kg relieved DSS-induced chronic colitis in mice by suppressing inflammation through compromised macrophage activity in colonic tissues associated with the colony-stimulating factor 1 receptor (Csf1r)/Src pathway. This evidence concerns the gene CSF1R and Chronic colitis.