In addition, close correlations between DAI, colon length, spleen weight, and gut microbiota were identified.<h4>Discussion</h4>Our findings revealed that the HLJD formula attenuated DSS-induced chronic colitis by reducing inflammation via Csf1r/Src-mediated macrophage infiltration, as well as modulating the gut microbiota profile. Here, CSF1R is linked to Chronic colitis.