This effect in DS samples was not sex-dependent; ROS production was increased significantly during reperfusion in mitochondria isolated from male Scn1a−/+ hearts, as well as (3.47 ± 0.61 in Scn1a+/+ vs. 10.22 ± 3.21 in Scn1a−/+; p = 0.03) female Scn1a−/+ hearts, ROS (5.27 ± 1.24 in Scn1a+/+ vs. 10.07 ± 1.65 in Scn1a−/+; p = 0.04) (Fig. 5C-D). This evidence concerns the gene SCN1A and Dravet syndrome.