The Masson’s trichrome staining results revealing the effects of CM and E2-CM on CTX-induced ovarian tissue fibrosis are presented in Fig. 2C. These results clearly indicate that ER+pcMSC-CM and E2-CM enhanced the proliferation and reduced the apoptosis of granulosa cells in the CTX-induced POI mice. The gene discussed is ESR1; the disease is cerebrotendinous xanthomatosis.