TRIM33 and esophageal squamous cell carcinoma: The results of membrane permeability JC-1 staining showed that, compared to the control group, shTRIM33 decreased the mitochondrial membrane potential, indicating that shTRIM33 promoted depolarization of the mitochondrial membrane and provided a basis for the early apoptosis of ESCC cells, which could be reversed by the overexpression of TRIM33 (Fig. 2E).