TRIM33 and esophageal squamous cell carcinoma: Consistent with the published data, quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that the level of TRIM33 mRNA in ESCC cell lines (TE-1, Eca109, and KYSE150) was higher than that in normal esophageal epithelial HEEC cells (Fig. 1D).