TRIM33 and esophageal squamous cell carcinoma: We subsequently performed Seahorse assays to monitor the intact cellular extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) in living cells, and silencing TRIM33 significantly impaired ECAR in ESCC cells, however, OCR levels significantly increased, which could be reversed by restoring TRIM33 expression (Fig. 3G, H).