To study the potential function of TRIM33 in the development of ESCC, we silenced the expression of endogenous TRIM33 in Eca109 and KYSE150 cells with lentiviral delivery of shRNAs and used a recombinant plasmid containing the pEnCMV-TRIM33-Myc gene to establish Eca109, KYSE150, and TE-1 cells overexpressing TRIM33. The gene discussed is MYC; the disease is esophageal squamous cell carcinoma.