The synthesized complexes,notably [Au(L3)(JohnPhos)] (3b), [Au(L4)(CyJohnPhos)](4a), and [Au(L4)(JohnPhos)] (4b), demonstratedsignificant antiproliferative activity against colon cancer cells,with a marked preference for cancerous cells over noncancerous ones.The anticancer effects of these complexes are primarily due to theirability to inhibit cyclooxygenase enzymes (COX-1/2), modulate reactiveoxygen species (ROS) levels by targeting thioredoxin reductase (TrxR),and induce apoptosis in cancer cells. This evidence concerns the gene PTGS1 and colonic neoplasm.