CD8A and breast cancer: 28 Because the entire field lacks an efficient, standardized, and in vitro approach to genetically manipulate non-model intestinal bacteria, such as BC, one limitation of the current research is that we were unable to silence ackA to block acetate synthesis in BC and thus establish a direct correlation between BC and acetate, which would enable us to dissect the specific role of BC-derived acetate for inducing optimal CD8+ T cell response and limiting IAV infection in vivo.