Transverse sections at the level of the SCO and caudal to the SCO in severely scoliotic fish revealed abnormally packed material in the ventricle (Figure 3N and Q, Figure 2—figure supplement 1J), while Sspo secreted cells from rpgrip1l-/- at scoliosis onset, that is in juveniles with a slight tail-up phenotype, appeared to produce Sspo-GFP cytoplasmic granules as in controls (Figure 2—figure supplement 1B, B’, D). This evidence concerns the gene SSPOP and scoliosis.