In addition, we found among the upregulated genes in dorsal trunk at scoliosis onset, a number of activated astrocyte markers (Matusova et al., 2023) such as s100b, c4b, gfap, glast/slc1a3b, viml, ctsb, (Figure 5—figure supplement 1D), showing that astrogliosis spread over the whole central nervous system. This evidence concerns the gene CTSB and scoliosis.