HCC was induced in mice using the hydrodynamic tail vein injection (HDTVi) model with the CRISPR/Cas9 system to knock out transformation-related protein 53 (Trp53) and with the Sleeping Beauty transposon system to overexpress c-Myc (Trp53KO c-MycOE). Here, TP53 is linked to hepatocellular carcinoma.