On the other hand, the CPS1‐induced upregulation of Asp could prevent invasion and metastasis of HCC cells by inhibiting the activity of phosphatidylcholine specific phospholipase C (PC‐PLC) to block the biosynthesis of diacylglycerol (DAG), leading to the downregulation of DAG‐PKC (protein kinase C) axis, which have been shown to promote invasion and metastasis of various cancer cells. The gene discussed is CPS1; the disease is cancer.