This was suspected based on a crumbled wing phenotype, resembling pex2 mutants.27 Indeed, we saw nuclear staining of YFP-PTS1, similar to Pex3 RNAi knockdown,26 a phenotype not as strong as when overexpressing X-ALD associated ABCD1R518Q. Here, PEX3 is linked to X-linked adrenoleukodystrophy.