Our studies found considerable evidence of immune dysfunction relative to healthy individuals in patients with CD.20 Specifically, we noted a significant increase in IL-2 expression; this pro-inflammatory cytokine is known for its high production by activated T cells.20 This discovery matches the existing literature, emphasizing the IL-2 role in controlling immune responses, especially concerning autoimmune disorders like CD.20 The abnormal expression of IL2 augments the activation of T-cells by gluten antigens, leading to an inflammatory cascade and gut inflammation in small intestines.44 This evidence concerns the gene IL2 and immune system disorder.