MAPK1 and major depressive disorder: Our study emphasizes the importance of “drug target-disease target” in the “component-target-pathway” approach by combining the KEGG pathway to retrieve all potential biomarkers associated with Kanye Goldenseal and MDD, and by using machine learning to screen and Identify MAPK1, EGF, HSP90AA1, AKT1, MYC as key targets for HP treatment of MDD.