Further strengthening the Rag-dependent separation of mTORC1 activities and showing that this is not a clonal artefact or a cell type-specific characteristic of the RagA/B KO HEK293FT cells, similar data were obtained using RagC/D KO HEK293FT cells (Extended Data Fig. 3a–c), RagA/B KO MEFs (Extended Data Fig. 3d–f) and RagA/B KO SW-620 colorectal cancer cells (Extended Data Fig. 3g). The gene discussed is RRAGC; the disease is colorectal cancer.