NR1D1 and malignant colon neoplasm: We then performed CRISPR knockout or shRNA/siRNA knockdown of the REV-ERBα/NR1D1 gene and found that its depletion significantly diminished the cell growth of castration-resistant prostate cancer (CRPC) (C4-2B, 22RV1, and LAPC4), HCC (Hepa1-6, HepG2, and Hep3B), and colon cancer (SW480, HCT116, and DLD-1) (Fig. 1 A and B and SI Appendix, Fig. S1 G and H).