In summary, successful isolation of uEVs by PEG-precipitation identified intact ACE2 and cleaved TMPRSS2 protein; while there were no sex-difference in ACE2 in uEVs as in tissue, TMPRSS2 protein was more abundant in uEVs from male patients with COVID-19 compared to healthy control males and female patients with COVID-19, respectively, in contrast to kidney tissue. Here, TMPRSS2 is linked to COVID-19.