In addition, compared with the normal group, the protein levels of GluR2 and 5‐HT2AR in the CA1 region of hippocampus of insomnia rats were significantly increased (p < 0.01), further increased in the GAD67‐KD group (p < 0.01), and decreased after senegenin intervention (p < 0.01); the expression was significantly decreased in the GAD67‐OE group (p < 0.01) and further decreased after senegenin intervention (p < 0.01). This evidence concerns the gene GAD1 and insomnia.