As shown in Figure 8, the expression of GAD65/67, GABA‐T, BDNF, and 5‐HT1AR protein levels was significantly decreased in CA1 region of hippocampus of insomnia rats (p < 0.01), further decreased after GAD67‐KD transfection (p < 0.01), and significantly increased after senegenin intervention (p < 0.01); the expression was significantly increased after GAD67‐OE transfection (p < 0.01) and further increased after senegenin intervention (p < 0.01). This evidence concerns the gene GAD2 and insomnia.