The results of the open field test showed that the activity time and total journey of insomnia rats were significantly reduced compared with the normal group (p < 0.01), and the spatial exploration ability of GAD67‐KD rats was impaired, resulting in a further reduction of activity time and total journey (p < 0.01), which was significantly prolonged after senegenin intervention (p < 0.01). The gene discussed is GAD1; the disease is insomnia.