GAD1 and insomnia measurement: GAD67 expression was significantly decreased after GAD67‐KD transfection compared with the insomnia group (p < 0.01), and significantly increased after KD + SEN intervention (p < 0.01); there was no difference in expression between GAD67‐OE transfection and insomnia group, and the expression was significantly increased after OE + SEN intervention (p < 0.01) (Figure 5c).