CHAT and synucleinopathy: To determine if seeded α-synucleinopathy induced a similar set of bioenergetic alterations in these neurons, aSYN PFFs were injected into the PPN of mice expressing Cre recombinase under the control of the promoter for choline acetyltransferase (ChAT) to allow cell-specific measurements to be performed (ChAT-Cre) (Fig. 4a; Fig. S9a).