The expression patterns across the 25 patients ranked according to NODAL expression identified a group of 16 patients with high NODAL positivity and a group of 9 patients with low NODAL positivity (with ≥45% and <45% of melanoma antigen-expressing tumor cells being positive for Nodal) based upon distinct alive/deceased and treatment response status (Figure 2A). Here, NODAL is linked to melanoma.