IL-1β expression was also evaluated in THP-1 macrophages after infection with the same strains for 3, 5 and 7 h; we were able to detect pro-IL1β only after infection with a reduction of 72% ± 0.09 after 3 h, 43% ± 0.1 after 5 h and 59% ± 0.04 after 7 h in cells infected with 93/4286ΩhrpB compared to 93/4286 strain (Figures 6A–C). The gene discussed is IL1B; the disease is infection.