These observations about the NSCLC cohort, which can be considered as limitations, are particularly relevant and should be taken into account when interpreting the results assessing whether clinical and demographical features such as age, sex, smoking status, histology category, EGFR mutational and PD-L1 positivity status contributed to TLS heterogeneity and correlated with our TLS Score. This evidence concerns the gene CD274 and non-small cell lung carcinoma.