However, the presence, phenotypes, and potential role of LDN in the pathogenesis of periodontitis have not yet been investigated.<h4>Objectives</h4>To investigate the presence, subsets (normal, band, suppressive, and active), and phenotypes via marker expression surface protein known as the cluster of differentiation (CD) (CD16b, CD14, CD15, and CD62L) of LDN in patients with periodontitis.<h4>Materials and methods</h4>The observational case-control study was conducted to estimate the potential role of LDNs in periodontitis. This evidence concerns the gene PROS1 and periodontitis.