Splice variant analysis of TCGA data showed that E26 and E27 are cassette exons of PBRM1; however, we did not observe any significant difference in the PSI values of PBRM1 E26 between normal and cancer tissues (Appendix Fig. S2A), leading us to focus only on E27 AS events in PBRM1. To validate TCGA results, PCR was performed using cDNA arrays of human cancer tissues with a primer pair targeting the E25 and E28 of PBRM1 (Appendix Fig. S2B). This evidence concerns the gene PBRM1 and cancer.