Subsequently, we selected eight genes (PBRM1, HERC2, KRAS, NF1, RNF213, NSD1, TRRAP, and CHD4) with the highest average |ΔPSI| scores, except for FN1 where AS site specification was difficult, and analysed the proportion of patients with cancer having AS changes in these genes by comparing a cancer tissue with an adjacent normal tissue derived from the same patient registered with TCGA database (Fig. 1B). This evidence concerns the gene NSD1 and cancer.