Immunohistochemical analysis confirmed distinct hormone synthesis profiles: the left adenoma diffusely expressed CYP11B2 without CYP11B1 expression, which was indicative of an aldosterone-producing adenoma, while the right adenoma exhibited diffuse expression of CYP11B1 and the absence of CYP11B2, which was consistent with a cortisol-secreting phenotype (Fig. 3). This evidence concerns the gene CYP11B1 and adenoma.