Infection of ATRX depleted cells with the ΔCTRL2 recombinant virus also yielded significantly higher IE gene expression over normal HFF cells at 1 hpi (Fig 11B) and finally, by comparison to wt, the gene expression defect was rescued back to the wt phenotype in the absence of ATRX (Fig 11C and 11D). This further stratifies mechanistic role for the CTRL2 insulator in ATRX deposition to the viral genome. Here, ATRX is linked to infection.