To further elucidate mechanisms of how the deletion of the CTRL2 insulator specifically influenced chromatin accessibility, we performed ChIP experiments to determine the enrichment of Alpha-thalassemia/mental retardation, X-linked chromatin remodeler (ATRX), and the histone variant H3.3, both of which are known for their roles in maintaining repressive histone markers on the viral genome. The gene discussed is ATRX; the disease is alpha thalassemia spectrum.