ACTA1 and neoplasm: Primary tumor and BCLM IMC markers in preserved clusters (Table 2) were associated with macrophages (CD68, CD163, and CD206), monocytes (CD14), immune response (CD56, CD4, and CD8a), PD1, PD-L1, tumor tissue (Ki-67 and phosphorylated ERK), cell adhesion (E-cad), hypoxia (HIF1α), vascularity (CD31), and ECM (αSMA, collagen, and MMP9).